QA-07Updated 2026-05-19
How are lyophilized research peptides reconstituted?
Lyophilized peptides are reconstituted by slowly adding bacteriostatic water (or another sterile, peptide-compatible solvent) down the inside of the vial wall, then gently swirling — never shaking — until fully dissolved.
Reconstitution is a routine lab procedure. The goal is to dissolve the peptide without denaturing it.
Standard protocol:
- Allow both the vial and the diluent to reach room temperature before opening.
- Use bacteriostatic water (0.9% benzyl alcohol) for most peptides intended for short-term refrigerated storage. Sterile water can be used for immediate single-use applications.
- Slowly inject the diluent down the inside wall of the vial — never directly onto the lyophilized cake.
- Swirl gently to dissolve. Do not shake; mechanical agitation can denature the peptide.
- Allow 60–90 seconds for full dissolution. The solution should be clear.
Concentration math is straightforward: divide the milligrams of peptide by the millilitres of diluent to get mg/mL.
Some peptides require a different solvent (acetic acid, DMSO, etc.) — always check the COA or product page. Reconstitution is part of research-protocol design; ADAM does not provide dosing guidance.